Intracellular staining of normal human peripheral blood cells that were unstimulated (orange histogram), stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81) and goat anti-mouse IgG for 5 minutes (purple histogram), or treated with hydrogen peroxide-activated sodium pervanadate (green histogram) with Anti-Human phospho-SLP-76 (Y128) APC. The Intracellular Fixation & Permeabilization Buffer Set (88-8824) and protocol were used for staining. Lymphocytes in the CD3+ gate were used for analysis.